Sperm subpopulation variability between stallion breeds

Sabrina Gacem, Jaime Catalán, Anthony Valverde Abarca, Jordi Miró, Carles Soler

Producción científica: Contribución a una revistaResumen de la jornadarevisión exhaustiva

Resumen

Computer Assisted Semen Analysis is as an objective method to identify sperm kinematic subpopulations related to semen quality, fertility, and freezability. The aim of this study is to compare the distribution of these sperm subpopulations between two horse breeds: Spanish (PRE), and Arabian (PRA). Two ejaculates were collected by artificial vagina from 13 PRE and 8 PRA and then diluted in
Kenney medium. Spermtrack® chambers of 10 µm depth were used for semen count. A minimum of 300 sperm were analyzed using a 250 frame rate. Principal component analysis and clustering procedures were performed to identify sperm subpopulations. After that, three well-defined subpopulations were characterized: SP1 (PRA:26%,PRE:24%) fast, straight and lineal sperm with a high beat; SP2 (PRA:40%, PRE:42%) fast but non-linear sperm with a high beat.
SP3 (PRA,PRE: 34%, each): slow and non-linear sperm subpopulation. The proportion of the three subpopulations for PRA and PRE breeds was closer but with a higher proportion of SP1 and lower
SP2 was found in PRA. However, the kinetic parameters inside the
same population showed a significant difference between breeds
with higher values for velocity parameters for PRE. In conclusion,
the analysis with a high frame rate permitted to evaluate the real
sperm trajectory and the right classification of different sperm kinematic subpopulations showing differences between breeds, where
PRE was faster and PRA more linear. It is necessary to differentiate among breeds to obtain useful results when evaluating stallion semen.
Idioma originalInglés
Número de artículoP22
Páginas (desde-hasta)43-129
PublicaciónReproduction in Domestic Animals
Volumen57
DOI
EstadoPublicada - 2021

Huella

Profundice en los temas de investigación de 'Sperm subpopulation variability between stallion breeds'. En conjunto forman una huella única.

Citar esto