CASA-Mot technology: How results are affected by the frame rate and counting chamber

Daznia Bompart, Almudena García-Molina, Anthony Valverde, Carina Caldeira, Jesús Yániz, Manuel Núñez De Murga, Carles Soler

Research output: Contribution to journalReview articlepeer-review

50 Scopus citations

Abstract

For over 30 years, CASA-Mot technology has been used for kinematic analysis of sperm motility in different mammalian species, but insufficient attention has been paid to the technical limitations of commercial computer-aided sperm analysis (CASA) systems. Counting chamber type and frame rate are two of the most important aspects to be taken into account. Counting chambers can be disposable or reusable, with different depths. In human semen analysis, reusable chambers with a depth of 10 μm are the most frequently used, whereas for most farm animal species it is more common to use disposable chambers with a depth of 20 μm. The frame rate was previously limited by the hardware, although changes in the number of images collected could lead to significant variations in some kinematic parameters, mainly in curvilinear velocity (VCL). A frame rate of 60 frames s -1 is widely considered to be the minimum necessary for satisfactory results. However, the frame rate is species specific and must be defined in each experimental condition. In conclusion, we show that the optimal combination of frame rate and counting chamber type and depth should be defined for each species and experimental condition in order to obtain reliable results.

Original languageEnglish
Pages (from-to)810-819
Number of pages10
JournalReproduction, Fertility and Development
Volume30
Issue number6
DOIs
StatePublished - 2018

Keywords

  • computer-aided sperm analysis (CASA) system
  • kinematic
  • motility.

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